catalog no 4917 Search Results


primary polyclonal anti stim2 antibody  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc primary polyclonal anti stim2 antibody
<t>STIM2</t> as the cornerstone of significantly increased SOCE in HD76 GABA MSNs. (A) Representative Western blot showing the expression level of STIM2 in HD76, HD76 incubated with 300 nM EVP4593 for 24 h and WT GABA MSNs. Band intensities were quantified and values normalized to the α-tubulin housekeeping protein. (B) Relative STIM2 levels in HD76 (red), HD76 incubated with 300 nM EVP4593 (green) and WT GABA MSNs (black). Biological replicates are mentioned above the bars. (C) Average Current-Voltage relationships (I–V curves) of normalized currents evoked by passive depletion of calcium stores with thapsigargin (1 μM) in HD76 expressing non-target shRNA (HD76 non-target shRNA, cyan line), HD76 expressing shRNA against STIM2 (HD76 STIM2(–), orange line), intact HD76 (red line) and HD76 pre-incubated with 300 nM EVP4593 for 24 h (green line). The number of experiments is depicted at the panel (D) . (D) Average amplitude or the normalized SOC currents determined at a test potential of −80 mV for HD76 expressing non-target shRNA (cyan), HD76 expressing shRNA against STIM2 (orange), intact HD76 (red) and HD76 pre-incubated with 300 nM EVP4593 for 24 h (green). The amplitudes are plotted as the mean ± SEM ( n = number of single cell experiments). The asterisk indicates that differences in amplitudes are statistically significant ( p < 0.05).
Primary Polyclonal Anti Stim2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/catalog+no+4917/STIM2+Antibody/pmc07884642-102-27-35
Average 93 stars, based on 1 article reviews
primary polyclonal anti stim2 antibody - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


STIM2 as the cornerstone of significantly increased SOCE in HD76 GABA MSNs. (A) Representative Western blot showing the expression level of STIM2 in HD76, HD76 incubated with 300 nM EVP4593 for 24 h and WT GABA MSNs. Band intensities were quantified and values normalized to the α-tubulin housekeeping protein. (B) Relative STIM2 levels in HD76 (red), HD76 incubated with 300 nM EVP4593 (green) and WT GABA MSNs (black). Biological replicates are mentioned above the bars. (C) Average Current-Voltage relationships (I–V curves) of normalized currents evoked by passive depletion of calcium stores with thapsigargin (1 μM) in HD76 expressing non-target shRNA (HD76 non-target shRNA, cyan line), HD76 expressing shRNA against STIM2 (HD76 STIM2(–), orange line), intact HD76 (red line) and HD76 pre-incubated with 300 nM EVP4593 for 24 h (green line). The number of experiments is depicted at the panel (D) . (D) Average amplitude or the normalized SOC currents determined at a test potential of −80 mV for HD76 expressing non-target shRNA (cyan), HD76 expressing shRNA against STIM2 (orange), intact HD76 (red) and HD76 pre-incubated with 300 nM EVP4593 for 24 h (green). The amplitudes are plotted as the mean ± SEM ( n = number of single cell experiments). The asterisk indicates that differences in amplitudes are statistically significant ( p < 0.05).

Journal: Frontiers in Cell and Developmental Biology

Article Title: STIM2 Mediates Excessive Store-Operated Calcium Entry in Patient-Specific iPSC-Derived Neurons Modeling a Juvenile Form of Huntington's Disease

doi: 10.3389/fcell.2021.625231

Figure Lengend Snippet: STIM2 as the cornerstone of significantly increased SOCE in HD76 GABA MSNs. (A) Representative Western blot showing the expression level of STIM2 in HD76, HD76 incubated with 300 nM EVP4593 for 24 h and WT GABA MSNs. Band intensities were quantified and values normalized to the α-tubulin housekeeping protein. (B) Relative STIM2 levels in HD76 (red), HD76 incubated with 300 nM EVP4593 (green) and WT GABA MSNs (black). Biological replicates are mentioned above the bars. (C) Average Current-Voltage relationships (I–V curves) of normalized currents evoked by passive depletion of calcium stores with thapsigargin (1 μM) in HD76 expressing non-target shRNA (HD76 non-target shRNA, cyan line), HD76 expressing shRNA against STIM2 (HD76 STIM2(–), orange line), intact HD76 (red line) and HD76 pre-incubated with 300 nM EVP4593 for 24 h (green line). The number of experiments is depicted at the panel (D) . (D) Average amplitude or the normalized SOC currents determined at a test potential of −80 mV for HD76 expressing non-target shRNA (cyan), HD76 expressing shRNA against STIM2 (orange), intact HD76 (red) and HD76 pre-incubated with 300 nM EVP4593 for 24 h (green). The amplitudes are plotted as the mean ± SEM ( n = number of single cell experiments). The asterisk indicates that differences in amplitudes are statistically significant ( p < 0.05).

Article Snippet: The membrane was incubated with 5% milk for 1 h at room temperature and treated with primary monoclonal anti-huntingtin antibody (1:5,000; catalog no. ab109115, Abcam, USA), or primary polyclonal anti-STIM2 antibody (1:1,000; catalog no. 4917, Cell Signaling Technology) and peroxidase-conjugated goat anti-mouse (1:30,000; catalog no. A0168, Sigma), or anti-rabbit (1:30,000; catalog no. A0545, Sigma, USA) IgG secondary antibody, respectively.

Techniques: Western Blot, Expressing, Incubation, shRNA

Disturbance of VGCC in HD76 neurons. (A) Average I–V curves of normalized voltage-gated calcium currents for HD76 (red circles) and WT (black squares) GABA MSNs. The number of experiments is depicted at the panel (B) . (B) Average amplitude of VGCC currents at the potential of −20 mV for HD76 (red), and WT (black) GABA MSNs. The amplitudes are plotted as the mean ± SEM ( n = number of single cell experiments). The asterisk indicates that differences in amplitudes are statistically significant ( p < 0.05). (C) Average I–V curves of normalized voltage-gated calcium currents in HD76 expressing non-target shRNA (HD76 non-target shRNA, cyan squares) and HD76 expressing shRNA against STIM2 (HD76 STIM2(–), orange circles). The number of experiments is depicted at the panel (D) . (D) Average amplitude of VGCC currents at the potential of −20 mV for HD76 expressing non-target shRNA (HD76 non-target shRNA, cyan) and HD76 expressing shRNA against STIM2 (HD76 STIM2(–), orange). The amplitudes are plotted as the mean ± SEM ( n = number of single cell experiments). n.s. indicates the absence of statistically significant differences ( p > 0.05).

Journal: Frontiers in Cell and Developmental Biology

Article Title: STIM2 Mediates Excessive Store-Operated Calcium Entry in Patient-Specific iPSC-Derived Neurons Modeling a Juvenile Form of Huntington's Disease

doi: 10.3389/fcell.2021.625231

Figure Lengend Snippet: Disturbance of VGCC in HD76 neurons. (A) Average I–V curves of normalized voltage-gated calcium currents for HD76 (red circles) and WT (black squares) GABA MSNs. The number of experiments is depicted at the panel (B) . (B) Average amplitude of VGCC currents at the potential of −20 mV for HD76 (red), and WT (black) GABA MSNs. The amplitudes are plotted as the mean ± SEM ( n = number of single cell experiments). The asterisk indicates that differences in amplitudes are statistically significant ( p < 0.05). (C) Average I–V curves of normalized voltage-gated calcium currents in HD76 expressing non-target shRNA (HD76 non-target shRNA, cyan squares) and HD76 expressing shRNA against STIM2 (HD76 STIM2(–), orange circles). The number of experiments is depicted at the panel (D) . (D) Average amplitude of VGCC currents at the potential of −20 mV for HD76 expressing non-target shRNA (HD76 non-target shRNA, cyan) and HD76 expressing shRNA against STIM2 (HD76 STIM2(–), orange). The amplitudes are plotted as the mean ± SEM ( n = number of single cell experiments). n.s. indicates the absence of statistically significant differences ( p > 0.05).

Article Snippet: The membrane was incubated with 5% milk for 1 h at room temperature and treated with primary monoclonal anti-huntingtin antibody (1:5,000; catalog no. ab109115, Abcam, USA), or primary polyclonal anti-STIM2 antibody (1:1,000; catalog no. 4917, Cell Signaling Technology) and peroxidase-conjugated goat anti-mouse (1:30,000; catalog no. A0168, Sigma), or anti-rabbit (1:30,000; catalog no. A0545, Sigma, USA) IgG secondary antibody, respectively.

Techniques: Expressing, shRNA